Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma

Abstract

Extracellular vesicles (EVs) in blood plasma offer a valuable reservoir of intracellular cellular cargo, making them a promising source of liquid-based biomarkers. However, the complexity of plasma, with its abundance of non-EV particles and plasma proteins, presents challenges for their molecular characterization, particular their lipid composition, using mass spectrometry–based technologies. Consequently, there is currently no comprehensive blueprint detailing both the proteomes and lipidomes of highly enriched plasma EVs. We employed an orthogonal approach using density gradient ultracentrifugation (DGUC) and size-exclusion chromatography (SEC) to isolate EVs and conducted a comparative s..